Photo-triggered fluorescent labelling of recombinant proteins in live cells.
نویسندگان
چکیده
A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed. The approach is based on photo-caged split-intein mediated conditional protein trans-splicing reaction and enabled background-free fluorescent labelling of target proteins with the necessary spatiotemporal control.
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ورودعنوان ژورنال:
- Chemical communications
دوره 51 47 شماره
صفحات -
تاریخ انتشار 2015